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Table 3 DNA extraction methods and quantification. Number and type of samples are here related with the extraction method used. Average amount of extracted DNA (ng/μl and per specimen) is shown

From: Description of the puparium and other notes on the morphological and molecular identification of Phthitia empirica (Diptera, Sphaeroceridae) collected from animal carcasses

Sample

Method

Elution (μl)

Average DNA quantification (ng/μl)

PCR success

1 adult

QIAamp® DNA

Investigator Kit

100

2.19 ± 0.02

✓

2 adults

QIAamp® DNA

Mini kit

200

0.93 ± 0.02

✓

3 close puparia

QIAamp® DNA

Mini kit

200

4.99 ± 0.02

✓

5 empty puparia

QIAamp® DNA

Investigator Kit

100

1.16 ± 0.01

X

  1. Standard deviation is reported. ✓positive PCR, X negative PCR result