Table 5 List of destructive techniques used in saliva analysis
From: Recent advancements in identification and detection of saliva as forensic evidence: a review
Techniques | Type of technique | Success rate |
|---|---|---|
Phadebas test | Destructive | It has many false positives since amylase is present in body fluids other than saliva as well |
SALIgAE test | Destructive | false-positive values have been traced in breast milk, rat saliva, clean urine, fecal/feces, and semen samples |
RSID | Destructive | Highly sensitive and rapid test |
Immunological test | Destructive | Multiple cross-reactivities are seen in this analysis |
RNA profiling | Destructive | The highly unstable nature of RNA causes hindrance |
DNA methylation | Destructive | The success rate of this technique is very high |
UV–Vis Spectroscopy | Destructive | Other body fluids often show similar ranges |
Microbial profiling | Destructive | The very high success rate on normal and degraded samples |
Polilight | Non-destructive | It has a low success rate as semen gives the same fluorescence as saliva, resulting in false positives |
Microscopic analysis | Non-destructive | Mixed samples cause variation in the concentration of metal |
Fluorescent spectroscopy | Non-destructive | Shows characteristic fluorescence for tryptophan |
Raman spectroscopy | Non-destructive | It has a good success rate |
FTIR spectroscopy | Non-destructive | Mixed and degraded samples do hinder the result |