Cytotoxicity associated with acute and chronic administration of synthetic cannabinoids “Strox” in the brain, liver, heart, and testes of male albino rats: histological and immunohistochemical study

Egyptian Journal of Forensic Sciences

Table 1 Immunohistochemical and degenerative changes scoring difference between the studied groups

	Negative control	Positive control	Acute treated	Chronic treated	Chronic withdrawal	p-value (between the groups)
	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	p-value (between the groups)
Immunohistochemical score	25.67 ± 4.5	21.56 ± 2.9	7.56 ± 2.3	0.67 ± 1	1.89 ± 1.4	≤ 0.001
P-value 1		0.23	≤ 0.001	≤ 0.001	≤ 0.001
P-value 2			≤ 0.001	≤ 0.001	≤ 0.001
P-value 3				≤ 0.001	0.001
P-value 4					0.878
Degenerative changes scoring (damage or shrinkage of cells)	0 ± 0	0.38 ± 0.5	1.88 ± 0.6	2.88 ± 0.35	2.75 ± 0.46	≤ 0.001
P-value 1			≤ 0.001	≤ 0.001	≤ 0.001
P-value 2			≤ 0.001	≤ 0.001	≤ 0.001
P-value 3				0.001	0.004
P-value 4					0.98

P1, comparison between negative control and positive control, acute, chronic, chronic withdrawal groups; P2, comparison between positive control and acute, chronic, chronic withdrawal groups; P3, comparison between acute treated group and chronic and chronic withdrawal groups; P4, comparison between chronic treated group and withdrawal group. SD, standard deviation, P-value < 0.05 considered statistically significant. ANOVA test was used to compare the mean difference between groups