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Table 2 A linear predicted equations and R2 values clarified the effect of different concentrations of clonazepam (25, 50, or 100 mg/ml), with respect to control samples, on different developmental stages (second, early third, and late third larval instars and prepupa) of Sarcophaga argyrostoma

From: Impact of using Sarcophaga (Liopygia) argyrostoma (Robineau-Desvoidy, 1830) as a toxicological sample in detecting clonazepam for forensic investigation

  

Stage

ra

p-value

R2

Equation

Type of equation

Morphological measurements

Weight

2nd stage

 − 0.34

 < 0.05

0.23

y =  − 0.007 ×  + 6.25

 

Early3rd stage

 − 0.29

 < 0.05

0.12

y =  − 0.015 ×  + 20.6

 

Late 3rd stage

0.15

 < 0.05

0.02

y = 0.089 ×  + 157.8

 

Prepupa

0.59

 < 0.05

0.29

y = 0.17 ×  + 141

Linear

equation for prediction

Length

2nd stage

0.91

 < 0.01

0.74

y = 0.01 ×  + 7.39

Early3rd stage

0.31

 < 0.05

0.05

y = 0.008 ×  + 7.9

Late 3rd stage

0.28

 < 0.05

0.02

y = 0.006 ×  + 17.1

Prepupa

0.51

 < 0.05

0.17

y = 0.012 ×  + 14.7

Width

2nd stage

0.21

 < 0.05

0.06

y = 0.001 ×  + 1.21

Early3rd stage

 − 0.33

 < 0.05

0.18

y =  − 0.002 ×  + 2.29

Late 3rd stage

0.58

 < 0.01

0.34

y = 0.004 ×  + 4.18

Prepupa

0.32

 < 0.05

0.08

y = 0.003 ×  + 3.91

Analytical analysis

HPLC analysis of clonazepam concentration

2nd stage

0.913

 < 0.01

0.91

y = 0.019 ×  + 3.41

Early3rd stage

0.954

 < 0.01

0.94

y = 0.021 ×  + 0.93

Late 3rd stage

0.939

 < 0.01

0.87

y = 0.011 ×  + 0.73

Prepupa

0.720

 < 0.01

0.81

y = 0.0019 ×  + 0.56

Biochemical analysis

Protein carbonyls amount

2nd stage

0.766

 < 0.01

0.57

y = 0.0031 ×  + 0.87

Early3rd stage

0.994

 < 0.01

0.98

y = 0.011 ×  + 0.70

Late 3rd stage

0.866

 < 0.01

0.74

y = 0.0037 ×  + 0.95

Prepupa

0.906

 < 0.01

0.81

y = 0.0049 ×  + 1.28

  1. aPearson correlation coefficient (two-tailed test)