Q1) Was a consecutive or random sample of patients enrolled?
Q2) Was a case control design avoided?
Q3) Did the study avoid inappropriate exclusions?
Q4) Were the index test results interpreted without knowledge of the results of the reference standard?
Q5) If a threshold was used, was it pre-specified?
Q6) Is the reference standard likely to correctly classify the target condition?
Q7) Were the reference standard results interpreted without knowledge of the results of the index test?
Q8) Was there an appropriate interval between index test and reference standard?
Q9) Did all patients receive the same reference standard?
Q10) Were all patients included in the analysis?