From: Development and validation of the method for the detection of glimepiride via derivatization employing N-methyl-N-(trimethylsilyl) trifluoroacetamide using gas chromatography-mass spectrometry
S. No.
Variables
Low level (-)
High level (+)
1.
Helium flow rate
1 ml/min
1.2 ml/min
2.
Injection port temperature
240 °C
250 °C
3.
Derivatization reaction time
50 min
60 min
4.
Derivatization reaction temperature
90 °C
100 °C