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Table 5 Comparison of the detected allele in STR profiles between different five bone samples processed with/without prior total demineralization process using the QIAGEN Investigator® IDplex Plus Kit

From: Factors influencing DNA extraction from human skeletal remains: bone characteristic and total demineralization process

Markers Bone A Bone B Bone C Bone D Bone E
I II I II I II I II I II
Amelogenin X,X X,X X,Y X,X X,Y X,– X,Y X,X X,– X,Y
TH01 7,9 10,10.3 6,7,9 9,9.3 5,7,10 6,7,9,10.3 7,7 13.3,– 6,– 7,9
D3S1358 15,18 16,– 16,17 16,17,18 10,15,16 11,15,16,17 15,–
vWA 14,14 16,– 17,– 13,– 14,– 16,18
D21S11
TPOX 8,– 8,– 8,8 8,9 8,15 8,9,11 8,9,11 8,9,11 8,9,10,11 8,–
D7S820 10,11 8,11 8,11,12 OL,13 8,OL 8,11,12
D19S433 13,14 14,– 14,– 14,16 6.2,13,15,17.2 13,– 15,–
D5S818 11,11 10,– 10,12
D2S1338
D16S539 9,12 10,– 9,– 9,9 10,11,13 9,10,11,12,13 11,12 10,11,12,13 11,12,13 9,10,11
CSF1PO 11,12 9,12 11,12 11,13 11,12 13,–
D13S317 10,11 8,9 10,12 8,12 9,–
FGA 21.2,– 19,– 22.2,–
D18S51 14,16 16,17 12,13,14 13,14,17,18.2,21.2 14,– 15,18
D8S1179 11,15,16 10,– 7,12,13,15,18 10,–
  1. I: Bone powder without total demineralization
  2. II: Bone powder with total demineralization (method of experimental group no. 9)
  3. OL off-ladder peak