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Table 5 Comparison of the detected allele in STR profiles between different five bone samples processed with/without prior total demineralization process using the QIAGEN Investigator® IDplex Plus Kit

From: Factors influencing DNA extraction from human skeletal remains: bone characteristic and total demineralization process

Markers

Bone A

Bone B

Bone C

Bone D

Bone E

I

II

I

II

I

II

I

II

I

II

Amelogenin

X,X

X,X

X,Y

X,X

X,Y

X,–

X,Y

X,X

X,–

X,Y

TH01

7,9

10,10.3

6,7,9

9,9.3

5,7,10

6,7,9,10.3

7,7

13.3,–

6,–

7,9

D3S1358

15,18

16,–

16,17

16,17,18

10,15,16

11,15,16,17

–

–

15,–

–

vWA

14,14

–

–

16,–

17,–

13,–

–

14,–

16,18

–

D21S11

–

–

–

–

–

–

–

–

–

–

TPOX

8,–

8,–

8,8

8,9

8,15

8,9,11

8,9,11

8,9,11

8,9,10,11

8,–

D7S820

10,11

–

–

8,11

8,11,12

OL,13

8,OL

–

8,11,12

–

D19S433

13,14

14,–

14,–

14,16

6.2,13,15,17.2

13,–

–

–

15,–

–

D5S818

11,11

–

10,–

–

10,12

–

–

–

–

–

D2S1338

–

–

–

–

–

–

–

–

–

–

D16S539

9,12

10,–

9,–

9,9

10,11,13

9,10,11,12,13

11,12

10,11,12,13

11,12,13

9,10,11

CSF1PO

11,12

–

9,12

11,12

11,13

11,12

–

13,–

–

–

D13S317

10,11

–

8,9

10,12

8,12

9,–

–

–

–

–

FGA

–

–

–

21.2,–

19,–

–

–

22.2,–

–

–

D18S51

14,16

–

16,17

12,13,14

13,14,17,18.2,21.2

14,–

15,18

–

–

–

D8S1179

–

–

11,15,16

10,–

7,12,13,15,18

–

–

10,–

–

–

  1. I: Bone powder without total demineralization
  2. II: Bone powder with total demineralization (method of experimental group no. 9)
  3. OL off-ladder peak