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Table 3 Estimated effect, regression coefficient, and ANOVA of the Plackett-Burman matrix of different components for calcium chelation by demineralization process

From: Factors influencing DNA extraction from human skeletal remains: bone characteristic and total demineralization process

Variables

Sum of squares

df

Coefficient

Mean squares

p value

Model

150,601.81

5

 

30,120.36

0.0007

Temperature

2043.63

1

13.05

2043.63

0.3002

Concentration

142,179.87

1

108.85

142,179.87

< 0.0001

pH

270.75

1

− 4.75

270.75

0.6960

Volume

5967.48

1

− 22.3

5967.48

0.0975

Time

140.08

1

3.416667

140.08

0.7781

Curvature

5449.85

1

 

5449.85

0.1105

Lack of fit

11,431.48

6

 

23,815.5799

0.0050

Coefficient of determination (R2) = 0.93

  1. df degree of freedom