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Table 1 Plackett-Burman experimental design matrix for the screening of various factors for total demineralization of skeletal remains. Thirteen experimental runs were generated using the Design-Expert software for five different factors

From: Factors influencing DNA extraction from human skeletal remains: bone characteristic and total demineralization process

Experimental groups

Factor 1, Temp (°C)

Factor 2, Conc (M)

Factor 3, pH

Factor 4, volume (mL)

Factor 5, time (h)

1

60

0.5

7

2

48

2

25

0.5

10

1

48

3

60

0.001

10

2

6

4

25

0.5

7

2

48

5

25

0.001

10

1

48

6

25

0.001

7

2

6

7

60

0.001

7

1

48

8

60

0.5

7

1

6

9

60

0.5

10

1

6

10

25

0.5

10

2

6

11

60

0.001

10

2

48

12

25

0.001

7

1

6

13

42.5

0.2505

8.5

1.5

27