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Table 1 Plackett-Burman experimental design matrix for the screening of various factors for total demineralization of skeletal remains. Thirteen experimental runs were generated using the Design-Expert software for five different factors

From: Factors influencing DNA extraction from human skeletal remains: bone characteristic and total demineralization process

Experimental groups Factor 1, Temp (°C) Factor 2, Conc (M) Factor 3, pH Factor 4, volume (mL) Factor 5, time (h)
1 60 0.5 7 2 48
2 25 0.5 10 1 48
3 60 0.001 10 2 6
4 25 0.5 7 2 48
5 25 0.001 10 1 48
6 25 0.001 7 2 6
7 60 0.001 7 1 48
8 60 0.5 7 1 6
9 60 0.5 10 1 6
10 25 0.5 10 2 6
11 60 0.001 10 2 48
12 25 0.001 7 1 6
13 42.5 0.2505 8.5 1.5 27