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Table 2 Observed STR profiles the Investigator® 24plex GO! for 22 reference samplesa. The profiles generated by GlobalFiler™ Express kit for 22 reference samplesb

From: Assessment of QIAGEN™ Investigator® 24plex GO! kit workflow for autosomal STR profiling of forensic reference samples

(a)
Allelic specificity 254805 254802 254803 254807 254808 254809 117095 142939 142962 142965 142972
D3S1358 A 16, 17 15, 16 15, 16 15, 16 16, 18 16, 18 14, 15 15, 16 15, 17 17, 17 16, 17
B 16, 17 15, 16 15, 16 15, 16 16, 18 16, 18 14, 15 15, 16 15, 17 17, 17 16, 17
vWA A 17, 17 16, 19 16, 19 17, 17 17, 18 17, 18 14, 17 14, 18 18, 19 17, 17 17, 18
B 17, 17 16, 19 16, 19 17, 17 17, 18 17, 18 14, 17 14, 18 18, 19 17, 17 17, 18
D16S539 A 10, 12 11, 12 11, 12 10, 14 11, 13 11, 13 11, 11 9, 12 10, 14 10, 11 9, 9
B 10, 12 11, 12 11, 12 10, 14 11, 13 11, 13 11, 11 9, 12 10, 14 10, 11 9, 9
CSF1PO A 10, 11 11, 11 11, 11 11, 13 12, 12 12, 12 12, 12 10, 11 12, 12 10, 11 12, 12
B 10, 11 11, 11 11, 11 11, 13 12, 12 12, 12 12, 12 10, 11 12, 12 10, 11 12, 12
TPOX A 8, 8 8.9 8, 9 8, 8 8, 8 8, 8 11, 11 8, 8 8, 8 8, 8 9, 9
B 8, 8 8.9 8, 9 8, 8 8, 8 8, 8 11, 11 8, 8 8, 8 8, 8 9, 9
Amelogenin A X, Y X, Y X, Y X, X X, X X, X X, Y X, Y X, Y X, Y X, Y
B X, Y X, Y X, Y X, X X, X X, X X, Y X, Y X, Y X, Y X, Y
D8S1179 A 12, 14 12, 15 12, 15 12, 15 13, 16 13, 16 10, 13 13, 16 13, 14 13, 15 13, 16
B 12, 14 12, 15 12, 15 12, 15 13, 16 13, 16 10, 13 13, 16 13, 14 13, 15 13, 16
D21S11 A 31, 32.2 28, 31 28, 31 28, 31 30, 32.2 30, 32.2 29, 32 29, 31.2 29, 32.2 27, 29 28, 31
B 31, 32.2 28, 31 28, 31 28, 31 30, 32.2 30, 32.2 29, 32 29, 31.2 29, 32.2 27, 29 28, 31
D18S51 A 14, 17 15, 15 15, 15 12, 16 14, 16 14, 16 14, 18 15, 15 14, 18 15, 17 14, 17
B 14, 17 15, 15 15, 15 12, 16 14, 16 14, 16 14, 18 15, 15 14, 18 15, 17 14, 17
DYS391 A 10, 10 10, 10 10, 10     10, 10 9, 9 10, 10 9, 9 10, 10
B 10, 10 10, 10 10, 10     10, 10 9, 9 10, 10 9, 9 10, 10
D2S441 A 11, 14 11.3, 12 11.3, 12 11, 14 11, 11 11, 11 12, 12 10, 10 10, 11 11, 11 12, 14
B 11, 14 11.3, 12 11.3, 12 11, 14 11, 11 11, 11 12, 12 10, 10 10, 11 11, 11 12, 14
D19S433 A 13, 13 12, 13 12, 13 14, 13 11, 13 11, 13 14, 16 12.2, 14 14, 14 14.2, 15 13, 14.2
B 13, 13 12, 13 12, 13 14, 13 11, 13 11, 13 14, 16 12.2, 14 14, 14 14.2, 15 13, 14.2
THO1 A 7, 8 9, 9 9, 9 9, 9 7, 8 7, 8 7, 9.3 8, 9 7, 8 6, 9 7, 9
B 7, 8 9, 9 9, 9 9, 9 7, 8 7, 8 7, 9.3 8, 9 7, 8 6, 9 7, 9
FGA A 21, 25 21, 25 21, 25 21, 22 23, 24 23, 24 19, 19 19, 23 19, 21 23, 26 22, 23
B 21, 25 21, 25 21, 25 21, 22 23, 24 23, 24 19, 19 19, 23 19, 21 23, 26 22, 23
D22S1045 A 11, 17 11, 15 11, 15 15, 15 11, 15 11, 15 15, 15 11, 15 11, 15 15, 16 11, 11
B 11, 17 11, 15 11, 15 15, 15 11, 15 11, 15 15, 15 11, 15 11, 15 15, 16 11, 11
D5S818 A 11, 11 11, 12 11, 12 10, 10 12, 13 12, 13 10, 13 11, 12 10, 11 10, 10 11, 12
B 11, 11 11, 12 11, 12 10, 10 12, 13 12, 13 10, 13 11, 12 10, 11 10, 10 11, 12
D13S317 A 11, 12 11, 12 11, 12 12, 12 10, 11 10, 11 8, 10 8, 8 12, 12 8, 11 11, 12
B 11, 12 11, 12 11, 12 12, 12 10, 11 10, 11 8, 10 8, 8 12, 12 8, 11 11, 12
D7S820 A 10, 12 11, 13 11, 13 12, 12 10, 11 10, 11 8, 10 11, 13 11, 11 10, 10 11, 11
B 10, 12 11, 13 11, 13 12, 12 10, 11 10, 11 8, 10 11, 13 11, 11 10, 10 11, 11
SE33 A 22.2, 27.2 26.2, 28 26.2, 28 18, 27.2 30.2, 30.2 30.2, 30.2 25.2, 29.2 23.2, 26.2 24.2, 26.2 19, 20 20, 26.2
B 22.2, 27.2 26.2, 28 26.2, 28 18, 27.2 30.2, 30.2 30.2, 30.2 25.2, 29.2 23.2, 26.2 24.2, 26.2 19, 20 20, 26.2
D10S1248 A 13, 15 15, 16 15, 16 14, 15 13, 15 13, 15 14, 15 13, 15 15, 17 12, 15 12, 15
B 13, 15 15, 16 15, 16 14, 15 13, 15 13, 15 14, 15 13, 15 15, 17 12, 15 12, 15
D1S1656 A 15, 16 15, 16.3 15, 16.3 12, 16 11, 15 11, 15 15, 17 15, 16 11, 16 11, 14 14, 15
B 15, 16 15, 16.3 15, 16.3 12, 16 11, 15 11, 15 15, 17 15, 16 11, 16 11, 14 14, 15
D12S391 A 18, 20 18, 20 18, 20 19, 22 19, 21 19, 21 20, 22 17, 20 17, 22 18, 19 23, 25
B 18, 20 18, 20 18, 20 19, 22 19, 21 19, 21 20, 22 17, 20 17, 22 18, 19 23, 25
D2S1338 A 20, 21 19, 22 19, 22 21, 23 22, 24 22, 24 17, 24 18, 19 23, 24 20, 24 20, 24
B 20, 21 19, 22 19, 22 21, 23 22, 24 22, 24 17, 24 18, 19 23, 24 20, 24 20, 24
(b)
Allelic specificity 148435 149824 149958 116846 116929 116935 116946 116956 117009 117022 117027
D3S1358 A 16, 17 15, 18 15, 16 17, 17 16, 16 17, 18 18, 18 14, 17 16, 17 17, 18 16, 17
B 16, 17 15, 18 15, 16 17, 17 16, 16 17, 18 18, 18 14, 17 16, 17 17, 18 16, 17
vWA A 15, 19 17, 18 14, 17 16, 18 16, 19 14, 16 17, 18 16, 16 17, 17 14, 17 14, 17
B 15, 19 17, 18 14, 17 16, 18 16, 19 14, 16 17, 18 16, 16 17, 17 14, 17 14, 17
D16S539 A 9, 10 10, 13 11, 13 12, 12 9, 11 11, 11 12, 13 10, 12 12, 13 10, 12 10, 12
B 9, 10 10, 13 11, 13 12, 12 9, 11 11, 11 12, 13 10, 12 12, 13 10, 12 10, 12
CSF1PO A 10, 12 12, 12 10, 10 11, 12 9, 9 12, 12 10, 10 10, 12 12, 12 10, 12 10, 11
B 10, 12 12, 12 10, 10 11, 12 9, 9 12, 12 10, 10 10, 12 12, 12 10, 12 10, 11
TPOX A 8, 9 8, 11 8, 8 9, 11 8, 10 8, 8 8, 9 8, 9 8, 8 8, 8 8, 10
B 8, 9 8, 11 8, 8 9, 11 8, 10 8, 8 8, 9 8, 9 8, 8 8, 8 8, 10
Amelogenin A X, Y X, Y X, Y X, Y X, Y X, Y X, Y X, Y X, Y X, Y X, Y
B X, Y X, Y X, Y X, Y X, Y X, Y X, Y X, Y X, Y X, Y X, Y
D8S1179 A 13, 13 10, 11 10, 15 11, 15 12, 13 13, 16 13, 13 11, 12 13, 15 14, 15 10, 13
B 13, 13 10, 11 10, 15 11, 15 12, 13 13, 16 13, 13 11, 12 13, 15 14, 15 10, 13
D21S11 A 30, 31 31, 32.2 29, 31 32.2, 33 29, 32.2 29, 31 29, 29 32.2, 32.2 31.2, 33.2 30, 31 28, 32.2
B 30, 31 31, 32.2 29, 31 32.2, 33 29, 32.2 29, 31 29, 29 32.2, 32.2 31.2, 33.2 30, 31 28, 32.2
D18S51 A 15, 17 13, 17 15, 23 14, 14 16, 20 14, 14 14, 16 19, 22 16, 17 15, 16 14, 19
B 15, 17 13, 17 15, 23 14, 14 16, 20 14, 14 14, 16 19, 22 16, 17 15, 16 14, 19
DY391 A 10, 10 10, 10 10, 10 11, 11 10, 10 11, 11 11, 11 10, 10 10, 10 10, 10 10, 10
B   10, 10 10, 10 11, 11 10, 10 11, 11 11, 11 10, 10 10, 10 10, 10 10, 10
D2S441 A 10, 10 11, 11 10, 14 10, 11 11.3, 14 11, 11.3 11, 11.3 11, 11 11, 12 12, 12 12, 14
B 10, 10 11, 11 10, 14 10, 11 11.3, 14 11, 11.3 11, 11.3 11, 11 11, 12 12, 12 12, 14
D19S433 A 13, 13 14, 15.2 13, 14.2 13, 15.2 13.2, 15 14, 15.2 15, 9 14, 15.2 13, 13.2 13.2, 15.2 12, 13
B 13, 13 14, 15.2 13, 14.2 13, 15.2 13.2, 15 14, 15.2 15, 9 14, 15.2 13, 13.2 13.2, 15.2 12, 13
THO1 A 7, 8 9.9 6, 9 7, 7 8, 9 8, 9 9, 9 7, 10 7, 7 7, 7 7, 7
B 7, 8 9.9 6, 9 7, 7 8, 9 8, 9 9, 9 7, 10 7, 7 7, 7 7, 7
FGA A 21, 22 19, 22 20, 22 19, 19 19, 24 19, 19 cOL 22, 23 22, 24 23, 26 19, 23
B 21, 22 19, 22 20, 22 19, 19 19, 24 19, 19 27.2 22, 23 22, 24 23, 26 19, 23
D22S1045 A 15, 16 11, 16 15, 16 15, 19 15, 17 11, 11 15, 17 15, 17 15, 16 17, 17 15, 16
B 15, 16 11, 16 15, 16 15, 19 15, 17 11, 11 15, 17 15, 17 15, 16 17, 17 15, 16
D5S818 A 10, 10 13, 14 10, 11 10, 12 10, 12 7, 9 12, 12 10, 12 9, 10 10, 10 11, 12
B 10, 10 13, 14 10, 11 10, 12 10, 12 7, 9 12, 12 10, 12 9, 10 10, 10 11, 12
D13S317 A 8, 12 9, 12 9, aOL 9, 12 8.8 10, 11 8, 8 10, 11 8, 11 8, 10 8, 8
B 8, 12 9, 12 9, cOL 9, 12 8.8 10, 11 8, 8 10, 11 8, 11 8, 10 8, 8
D7S820 A 11, 11 8, 10 11, 13 10, 12 10.1, 11 10, 13 8, 11 10, 10 10, 11 10, 10 11, 11
B 11, 11 8, 10 11, 13 10, 12 10.1, 11 10, 13 8, 11 10, 10 10, 11 10, 10 11, 11
SE33 A 21, 30.2 27.2, 27.2 26.2, 31.2 19, 27.2 20, 22.2 21, 26.2 22.2, 25.2 20, 30.2 25.2, 26.2 20, 22.2 21, 30.2
B 21, 30.2 27.2, 27.2 26.2, 31.2 19, 27.2 20, 22.2 21, 26.2 22.2, 25.2 20, 30.2 25.2, 26.2 20, 22.2 21, 30.2
D10S1248 A 13, 14 13, 13 15, 15 13, 15 14, 15 13, 14 13, 15 13, 15 13, 14 16, 16 14, 16
B 13, 14 13, 13 15, 15 13, 15 14, 15 13, 14 13, 15 13, 15 13, 14 16, 16 14, 16
D1S1656 A 12, 16 16, 17 13, 15 11, 16.3 15, 17 12, 16 13, 15 14, 17.3 12, 13 15, 17 14, 15
B 12, 16 16, 17 13, 15 11, 16.3 15, 17 12, 16 13, 15 14, 17.3 12, 13 15, 17 14, 15
D12S391 A 18, 20 19, 19 19, 24 18, 19 10, 10 19, 19 17, 23 21, 23 19, 22 18, 18 17, 21
B 18, 20 19, 19 19, 24 18,19 20, 23 19, 19 17, 23 21, 23 19, 22 18, 18 17, 21
D2S1338 A 22, 23 16, 22 17, 19 23, 25 20, 23 16, 18 23, 24 17, 24 20, 23 20, 22 20, 23
B 22, 23 16, 22 17, 19 23, 25 20, 23 16, 18 23, 24 17, 24 20, 23 20, 22 20, 23
  1. cOL Off ladder