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Table 1 Analytical threshold (AT) calculation

From: Assessment of QIAGEN™ Investigator® 24plex GO! kit workflow for autosomal STR profiling of forensic reference samples

Sample control ID Threshold channel
Blue Green Yellow Red Purple
NC 1 39 37 38 57 31
NC 2 23 21 18 30 64
NC 3 21 22 20 33 36
NC 4 23 25 19 26 34
NC 5 29 36 23 29 31
NC 7 58 34 25 22 24
NC 8 31 23 18 24 23
NC 9 31 22 21 23 22
NC 10 24 23 22 25 26
NC 11 47 38 23 38 38
NC 12 22 22 22 38 36
NC 13 43 37 25 29 24
NC 14 42 34 17 52 33
NC 15 31 24 24 31 27
NC 16 31 16 17 30 29
NC 17 41 28 29 55 43
NC 18 40 32 20 44 32
NC 19 39 24 21 70 29
Highest RFU 58 38 38 70 64
Average 34.68 28.00 22.58 37.11 32.84
Std. Dev. 10.09 6.84 4.99 13.67 9.70
aLOD 64.95 48.52 37.56 78.13 61.95
bLOQ 135.57 96.39 72.50 173.85 129.87
  1. aAverage + 3SD
  2. bAverage + 10SD
  3. Analytical threshold for size marker was set to 50 RFU and AT = 3 × LOQ