Egyptian Journal of Forensic Sciences

Table 1 Analytical threshold (AT) calculation

From: Assessment of QIAGEN™ Investigator® 24plex GO! kit workflow for autosomal STR profiling of forensic reference samples

Sample control ID	●Threshold channel
Sample control ID	Blue	Green	Yellow	Red	Purple
NC 1	39	37	38	57	31
NC 2	23	21	18	30	64
NC 3	21	22	20	33	36
NC 4	23	25	19	26	34
NC 5	29	36	23	29	31
NC 7	58	34	25	22	24
NC 8	31	23	18	24	23
NC 9	31	22	21	23	22
NC 10	24	23	22	25	26
NC 11	47	38	23	38	38
NC 12	22	22	22	38	36
NC 13	43	37	25	29	24
NC 14	42	34	17	52	33
NC 15	31	24	24	31	27
NC 16	31	16	17	30	29
NC 17	41	28	29	55	43
NC 18	40	32	20	44	32
NC 19	39	24	21	70	29
Highest RFU	58	38	38	70	64
Average	34.68	28.00	22.58	37.11	32.84
Std. Dev.	10.09	6.84	4.99	13.67	9.70
^aLOD	64.95	48.52	37.56	78.13	61.95
^bLOQ	135.57	96.39	72.50	173.85	129.87

^aAverage + 3SD
^bAverage + 10SD
^●Analytical threshold for size marker was set to 50 RFU and AT = 3 × LOQ

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