Table 1 Histological and microbiological findings among the various study groups
Group | Extracellular matrix | Cellular components | Microbiological component | ||||
|---|---|---|---|---|---|---|---|
Integrity of extracellular matrix | Vacuolations in the extracellular matrix | Fibroblasts | Odontoblastic layer | Defense cells | Endothelial cells and RBCs | Bacterial status | |
Group 1 | Homogeneous fibrous matrix | Few small vacuolations | Plenty in number with basophilic spindle-shaped nuclei with homogeneous chromatin. No signs of degeneration seen | Peripheral odontoblastic layer with numerous palisading odontoblasts present | Numerous scattered defense cells with deeply basophilic nucleus | Blood vessels with endothelial cell lining engorged with RBCs seen. | Gram positive Staphylococci and Streptococci seen |
Group 2 | Loose fibrous with no disintegration of the intercellular matrix | Numerous small vacuolations | Numerous fibroblasts with basophilic spindle-shaped nuclei containing homogeneous chromatin. No signs of degeneration seen | Odontoblastic layer intact | Numerous scattered defense cells with light stained basophilic nucleus | Blood vessels with few endothelial cells having less number of RBCs. | Gram positive Staphylococci and Streptococci seen |
Group 3 | Almost half of the matrix degeneration seen. | Numerous large vacuolated areas | Reduced cellularity with nucleus showing signs of karyorrhexis | Odontoblastic layer intact | Focal areas of defense cells seen with light stained basophilic nucleus | Endothelial cells show degeneration and debris of degenerated RBCs present | Gram-positive Staphylococci and Streptococci seen |
Group 4 | Strands of matrix seen at the center and periphery | Large vacuoles filling the stroma | Considerably less cellular with nucleus showing signs of karyorrhexis and pyknosis | Odontoblastic layer seen in few areas at the periphery | Scanty defense cells with light stained basophilic nucleus | Not comprehendible | Gram-positive Staphylococci and Streptococci seen |
Group 5 | Strands of matrix seen only at periphery | Large areas of vacuoles filling the entire stroma | Negligible number of fibroblasts with nucleus showing signs of pyknosis | Odontoblastic layer seen in few areas at the periphery | Defense cells negligible with pyknotic nucleus | Not comprehendible | Gram-positive Staphylococci and Streptococci seen |
Group 6 | Strands of collagen matrix seen only at periphery | Predominantly vacuolated stroma | Few fibroblasts were seen | Odontoblastic layer seen in few areas | Not comprehendible | Not comprehendible | Gram positive Staphylococci and Streptococci seen |
Group 7 | Thin strands of collagen matrix seen only at periphery | Separation of stroma | Nuclear debris seen | Absent | Not Comprehendible | Not comprehendible | Gram-positive Staphylococci and Streptococci seen |
Group 8 | Thin lightly eosinophilic streaks of collagen seen | Separation of stroma | Absent | Absent | Not comprehendible | Not comprehendible | Gram positive Staphylococci and Streptococci seen |