Table 1 The total ion chromatogram (m/z) and quality (%) of heroin metabolite measured in larvae, pupae and adult stages of L. cuprina reared in meat containing different concentrations of heroin. m/z represents mass divided by change number and the horizontal axis in a mass spectrum is expressed in units of m/z
Time (hour) | Stages | Control | 500 ng/μl | 1000 ng/μl | 2500 ng/°° | 5000 ng/μl | 10,000 ng/μl | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Retention time (m/z) | Quality (%) | Metabolite | Retention time (m/z) | Quality (%) | Metabolite | Retention time (m/z) | Quality (%) | Metabolite | Retention time (m/z) | Quality (%) | Metabolite | Retention time (m/z) | Quality (%) | Metabolite | |||
8 h–24 h | 1st instar larvae | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |
32 h–56 h | 2nd instar larvae | ND | 3.75 | 16 | Tryptophan-M ME | 4.78 | 25 | Tryptophan-M ME | 3.76 | 27 | Tryptophan-M ME | 3.61 | 11 | Morphine-M ME | 3.61 | 95 | Morphine |
64 h–224 h | 3rd instar larvae | ND | 8.06 | 1 | Hydromorphone enol 2TMS | 3.32 | 9 | Hydromorphone enol 2TMS | 3.74 | 53 | Tryptophan-M ME | 4.94 | 96 | Morphine | 4.94 | 96 | Morphine |
232 h–280 h | Pupae | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND | ND |