Fig. 4

Representative H&E stained sections from the cortex of rats treated with a saline; b cannabis 5 mg/kg showing pyknotic darkly stained nuclei (P); c cannabis 10 mg/kg showing cytoplasmic vacuolations (V) and pyknotic darkly stained nuclei (P); d cannabis 20 mg/kg showing (left) cytoplasmic vacuolations (V), pyknotic nuclei (P), and apoptotic cells (A) and (right) hemorrhage (H) and focal gliosis (arrow); e Tramadol 5 mg/kg showing cytoplasmic vacuolations (V), pyknotic nuclei (P), and apoptotic cells (A); f Tramadol 10 mg/kg showing pyknotic nuclei (P) and hemorrhage (H); g Tramadol 20 mg/kg showing (left) cytoplasmic vacuolations (V), hemorrhage (H), and pyknotic nuclei (P) and (right) mild diffuse gliosis (arrow); h cannabis 5 mg/kg + tramadol 10 mg/kg showing cytoplasmic vacuolations (V), pyknotic nuclei (P), and hemorrhage (H); i cannabis 10 mg/kg + tramadol 10 mg/kg showing pyknotic nuclei (P); j cannabis 20 mg/kg + tramadol 10 mg/kg showing (left) cytoplasmic vacuolations (V), pyknotic nuclei (P), and apoptotic cells (A) and (right) focal gliosis (arrow) (H&E × 400)