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Table 1 Sensitivity of three semen detection tests. Positive or negative scores are shown for each donor (males 1–5)

From: Integrating presumptive and confirmatory semen tests into DNA profiling of sexual assault evidence: a Philippine example

Concentration of semen (% v/v)a

ALS

AP

Sg

1

2

3

4

5

1

2

3

4

5

1

2

3

4

5

100

+

+

+

+

+

+

+

+

+

+

n.d.

n.d.

n.d.

n.d.

n.d.

50

+

+

+

+

+

+

+

+

+

+

n.d.

n.d.

n.d.

n.d.

n.d.

25

+

+

+

+

+

+

+

+

+

+

n.d.

n.d.

n.d.

n.d.

n.d.

12.5

+

+

+

+

+

+

+

+

+

+

+

+

+

+

+

6.25

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

+

+

+

+

+

5

+

+

+

+

+

+

+

+

+

3.125

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

+

+

+

+

+

1.5625

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

+

+

+

+

+

0.8929

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

+

+

+

+

+

0.5

+

+

+

+

+

+

0.05

+

+

+

+

+

0.005

+

+

+

+

+

0.0005

+

+

0.00005

0b

  1. n.d. not done (as different series of diluted semen were prepared separately for ALS/AP and Sg tests, not all concentrations listed were applicable to the tests)
  2. aSemen lost due to < 100% efficiency of UB extraction was not accounted for in the computation
  3. bThis represents the negative control where PBS was used for the ALS/AP tests and UB for the Sg test