Table 1 The species-specific 12S rRNA and short tandem repeats (STRs) primer sequences, references and amplicon sizes used in meat product authentication
Species name and DNA specific markers | Forward and reverse primers (primer sequence 5′-3′) | Amplicons (bp) | References |
|---|---|---|---|
Chicken (12S rRNA) | F 5′-TGAGAACTACGAGCACAAAC-3′ R 5′-GGGCTATTGAGCTCACTGTT-3′ | 183 bp | Dalmasso et al. 2004 |
Donkey (12S rRNA) | F: 5’-TGCTAGCCTCATTATCAGTAT-3′ R: 5′-GTGATGAGGATACGTGCT-3′ | 83 bp | Kesmen et al. 2009 |
Human (TPOX)a Repeat [AATG] | F: 5′-ACT GGC ACA GAA CAG GCA CTT AGG-3′ R: 5′-GGA GGA ACT GGG AAC CAC ACA GGT-3′ | 216–264 bp | Butler and Reeder, 2018 |
Human (D18S51)b Repeat [GAAA] | F: 5′-CAA ACC CGA CTA CCA GCA AC-3′ R: 5′-GAG CCA TGT TCA TGC CAC TG-3′ | 262–349 bp |