Concentration and Purity DNA Spectrophotometer: Sodium Monofluorophosphate forensic impended effect

Egyptian Journal of Forensic Sciences

Table 1 Concentration and Purity of DNA obtained from measured samples in relation with the effect of SMFP

Sample code	Sample sex	OD₂₆₀ (nm)	OD₂₈₀ (nm)	DNA concentration (μg/ml)	DNA purity
“a”	Female	0.106	0.085	371	1.25
“b”	Female	0.044	0.033	154	1.33
“c”	Male	0.016	0.007	56	2.29
“d”	Male	0.025	0.016	87.5	1.56
“e”	Male	0.032	0.021	112	1.52
“f”	Male	0.048	0.035	161	1.37
“g”	Male	0.029	0.018	101.5	1.61
“h”	Male	0.024	0.016	84	1.5
“i”	Female	0.056	0.041	196	1.37
“j”	Female	0.039	0.027	136.5	1.44

Ascertained implication was assessed by establishing quality and quantity parameters to support the suggested effect of SMFP. The spectrophotometer measured parameters: concentration and purity corresponded to the degree of toothpaste remains in the toothbrush. Greenish coloured sample “a” presented the highest amount of DNA concentration (371 μg/ml) under OD₂₆₀ (nm) Absorbance while in return, purity measured the lowest level (1.25) of other samples signifying a lost DNA intactness by SMFP. Sample “a” shows that the SMFP effect increases as the amount of compounded toothpaste increases