Fig. 4
From: Crime investigation through DNA methylation analysis: methods and applications in forensics
Schematic representation of Methyl-sensitive Single Nucleotide Primer Extension (Ms-SNuPE) method. a This method can be used to detect cytosine methylation by designing primers of different lengths corresponding to different cytosine markers that vary among body fluids/tissues of human (e.g. blood, saliva, and semen) or between monozygotic twins or with the ageing of human beings. The primers are extended a single base with fluorescently labeled terminators (ddNTPs-Fluorophore) using DNA polymerase b The extended primers are then detected by running these products through the genetic analyzer (capillary electrophoresis)