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Fig. 2 | Egyptian Journal of Forensic Sciences

Fig. 2

From: Crime investigation through DNA methylation analysis: methods and applications in forensics

Fig. 2

Bisulfite sequencing, Methylation Specific PCR and Restriction Analysis. Methylation status of cytosines of two different samples/alleles (one methylated and another unmethylated) is detected and confirmed by bisulfite sequencing. For the genomic level of 5mC analysis, bisulfite treatment can be followed by next generation sequencing (BS-seq) method. The data obtained from bisulfite sequencing can be pursued further for Methylation Specific PCR (MSP), Combined BS Restriction Analysis (COBRA) and Methylation-sensitive Single Nucleotide Primer Extension (Ms-SNuPE, explained in Fig. 4) for routine analysis of specific cytosine residues

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