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Table 2 PCR program for amplification of AR, SRY and DYS14 genes

From: Sex determination from dental pulp DNA among Egyptians

Steps PCR Program for AR gene PCR Program for SRY gene PCR Program for DYS14 gene
Initial denaturation 95 °C for 10 mins 95 °C for 10 mins 95 °C for 10 mins
Denaturation 95 °C for 20 s 95 °C for 1 min. 95 °C for 30 s
Annealing 72 °C for 1 min.a 58 °C for 1 min. 60 °C for 30 s
Extension   72 °C for 1 min. 72 °C for 30 s
Number of cycles 35 cycles 35 cycles 35 cycles
Final extension 72 °C for 7 mins 72 °C for 10 mins 72 °C for 10 mins
  1. aAnnealing and extension steps are combined together in one step