Table 2 PCR program for amplification of AR, SRY and DYS14 genes
From: Sex determination from dental pulp DNA among Egyptians
Steps | PCR Program for AR gene | PCR Program for SRY gene | PCR Program for DYS14 gene |
|---|---|---|---|
Initial denaturation | 95 °C for 10 mins | 95 °C for 10 mins | 95 °C for 10 mins |
Denaturation | 95 °C for 20 s | 95 °C for 1 min. | 95 °C for 30 s |
Annealing | 72 °C for 1 min.a | 58 °C for 1 min. | 60 °C for 30 s |
Extension | 72 °C for 1 min. | 72 °C for 30 s | |
Number of cycles | 35 cycles | 35 cycles | 35 cycles |
Final extension | 72 °C for 7 mins | 72 °C for 10 mins | 72 °C for 10 mins |