Table 1 Optimized conditions for HPLC

Mobile phase 1 (Bottle C)

Acetonitrile

Mobile phase 2 (Bottle D)

2 mM ammonium formate

Mode of mobile phase

Isocratic 50:50 C:D

Flow rate

1 mL/min

Degasser

Normal

Column temperature

30°C ± 5 °C

Autosampler temperature

20°C ± 5 °C

Injection volume

25 μL

Mode of data acquisition

3D data collection

Scan range

190 to 400 nm

Resolution

1.2

Sampling rate

1.0

UV max detection for quantification

210 nm

Total run time^a

18–25 min